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. Author manuscript; available in PMC: 2012 Jul 15.
Published in final edited form as: Exp Cell Res. 2011 Mar 21;317(12):1736–1745. doi: 10.1016/j.yexcr.2011.03.004

Fig. 4.

Fig. 4

Influence of tamoxifen on intracellular levels of C6-ceramide and on synthesis on long-chain ceramide from C6-ceramide in 2780AD cells. (A) Influence of tamoxifen on intracellular C6-ceramide levels. Cells in 6-well plates were exposed to [14C]C6-ceramide (5 μg/ml medium) in the absence and presence of tamoxifen (5 μM) for 24 hr. [14C]C6-ceramide was isolated from the total cellular lipid extracts by TLC and quantified by LSC. (B) Influence of tamoxifen on synthesis of long-chain ceramide from C6-ceramide. Cells in 6-well plates were cultured as indicated (unlabeled C6-ceramide, 5 μg/ml; tamoxifen 5 μM) in medium containing 1.0 μCi/ml [3H]palmitic acid for 24 hr. 3H-Ceramide was isolated and quantitated from total cellular lipid extracts by TLC and LSC. Results represent mean ± S.D., n = 3 cultures/condition.