Fig. 2.
Time course of apoptosis of neutrophils, which have transmigrated through HUVEC. Comparisons were made between freshly isolated neutrophils (▲) and neutrophils that had migrated for 2 h through unstimulated HUVEC (□) or through HUVEC that had been treated with 1 U/ml TNF (△) or 100 U/ml TNF (■). Data are the mean ± sem from three of more independent experiments, except at 72 h, where n = 2. ANOVA showed significant effects of time and treatment on apoptosis (P<0.01 in each case).