Figure 5.
Injection of Lactobacilli-treated DCs into NOD mice confers some protection. Six-week-old NOD mice received two injections of 1 × 106 DCs treated with high dose (20 × 106 CFU/mL; NOD-high LC) of LC at 6 and 16 weeks of age (n = 4–6) and blood glucose was measured weekly and diabetes incidence determined (a). Sera from NOD mice uninjected or injected with DCs treated with high-dose LC (high LC) were collected at 28 hrs after injection, and tested for the presence of IL-10 by ELISA (b). Six-week-old NOD mice left uninjected or injected with 1 × 106 untreated DCs (n = 4-5) or DCs treated with high dose of LC (n = 4) received anti-IL-10R or isotype control antibodies, and blood glucose was measured weekly and diabetes incidence determined (c). *indicates a significant difference at P < .03.