Table 3.
Effects of rec12-G202E upon intragenic (gene conversion) recombination at the ade6 locus
| Strains crossed | Genotype at rec12 locus | Recombinant frequency (× 106) |
|---|---|---|
| WSP0016 × WSP0578 | +/+ | 536 ± 113 |
| WSP1828 × WSP1829 | D15/D15 | 9.09 ± 3.42 |
| WSP1804 × WSP0346 | G202E/G202E | 4.06 ± 2.99 |
Spores were plated on nonselective and selective media to determine the viable titer and the adenine prototrophic titer, respectively. The recombinant frequency (RF) was calculated as [(recombinant titer)/(total viable titer)]. Data are mean ± standard deviation of RF values from three separate experiments.