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Preparation of genomic DNA inserts for construction of libraries. (A) EtBr-stained, agarose gel analysis of genomic DNA digested with different concentrations of Sau3AI. (B) Analysis of pooled, partial-digest genomic DNA prior to and after size fractionation. Size-fractionated samples were cloned into the BamHI restriction sites of pBluescript KS+ and pFY20, respectively
