. Author manuscript; available in PMC: 2011 Jun 21.

Published in final edited form as: Biochemistry. 2005 Aug 2;44(30):10298–10304. doi: 10.1021/bi050638t

Table 2.

Binding constants for factor VIII reagents to activated platelets

Ligand	Number of Binding Sites (per platelet)	Apparent K_d (nM)
wild type FVIII	627 ± 48	1.68 ± 0.43
E113A FVIII	750 ± 46 (p>0.05)^*	0.86 ± 0.23 (p> 0.05)
wild type FVIIIa	963 ± 75	2.51 ± 0.55
E113A FVIIIa	1083 ± 59 (p>0.05)	1.20 ± 0.25 (p<0.05)

Parameter values were estimated by nonlinear least square regression of the data shown in Figures 5A and 5B.

Statistical significance of the difference of the values between wild type and E113A was calculated by Student s t-test.