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Transcription regulation of the Y. pestis galETK operon. In vitro transcription assays were performed on plasmid templates containing the regulatory region of the gal operon from E. coli (A) and from Y. pestis (B). Lane 2 shows the effect of GalRYP, while lane 3 shows the combined effect of GalRYP and HU. Protein concentrations are indicated below the lanes. The RNA1 transcript, which does not vary with the GalR concentration, was used as an internal control between lanes.
