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. Author manuscript; available in PMC: 2011 Jun 21.
Published in final edited form as: J Control Release. 2009 Oct 3;141(2):193–198. doi: 10.1016/j.jconrel.2009.09.017

Table 1.

The fluorescence of FITC-ODN and FITC-ODN/Iowa Black FQ-ODN at differing pH’s. FITC-ODN was present at 0.05 μM in the absence or presence of 0.10 μM Iowa Black FQ-ODN. The fluorescence of FITC-ODN or FITC-ODN/Iowa Black FQ-ODN at neutral pH (control) was taken as 1.0 for determining relative fluorescence at other pH values. “Time” (last column) refers to the time to attain a stable fluorescence signal demonstrating how long it takes to complete hybridization of ODN.

pH Fraction of Initial FITC-ODN Fluorescence Fraction of FITC-ODN/Iowa Black FQ-ODN Fluorescence % Quench Time (mins)
7.2 (control) 1.0 1.0 87±0.2 8
8.5 1.20±0.02 2.0±0.09 85±0.4 17
12.2 1.40±0.08 19.1±0.50 3.0±2.6 25