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. Author manuscript; available in PMC: 2012 Apr 19.
Published in final edited form as: Biochemistry. 2011 Mar 25;50(15):3193–3203. doi: 10.1021/bi200037j

Figure 3.

Figure 3

Anionic lipid enhances extra MII stabilization. Quantiation of extra-metarhodopsin II in the presence of different negatively charged bicelles. Extra-metarhodopsin II was assessed in the presence of (a) DMPS or (b) DMPA containing bicelles. The ratio of neutral to negatively charged phospholipids, DMPC:DMPS (or DMPA), was 97:3, 70:30 or 50:50 in these bicelles. The final concentration of bicelles was 8%. The average lipid:rhodopsin ratio was 12800:1. Data were collected at 4 °C and normalized to the extra-metarhodopsin signal measured in ROS membranes under the same conditions. Results are mean ± S.E.M. values of three independent experiments (* p < 0.05; ** p < 0.01; *** p < 0.001; NS, Not Significant).