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. Author manuscript; available in PMC: 2012 Apr 19.
Published in final edited form as: Biochemistry. 2011 Mar 25;50(15):3193–3203. doi: 10.1021/bi200037j

Figure 7.

Figure 7

Stability of rhodopsin-Gt(empty) complex in the presence of negatively charged bicelles. The effect of negatively charged bicelles on complex stability was evaluated at (a) 4 °C and (b) 15 °C. (c) The effect of pH on complex stability at 4 °C (black bars) and at 15 °C (grey bars) in the presence of PA+PS (70:30) bicelles. Final concentrations of PA+PS (70:30) bicelles were held constant at 8% (lipid:rhodopsin ratio of approximately 12800:1). The ratio of neutral to negatively charged phospholipids is indicated within the parentheses in the graph. The half life of extra MII signal was calculated by using an exponential decay equation. Results are mean ± S.E.M. values from at least three independent experiments (* p < 0.05; ** p < 0.01; *** p < 0.001; NS, Not Significant).