Table 2.

Sequence and features of PCR

Gene (gene bank ID)	Primer sequence (5′ to 3′)	Annealing Tm (°C)	Product size (bp)
MT-1A (NM_005946)
Forward:	ATGGACCCCAACTGCTCCTGC	59	177
Reverse:	CAGCTGCACTTCTCTGATGCCC
β-actin (NM_001101)
Forward:	TTCTACAATGAGCTGCGTGTGG	59	119
Reverse:	GTGTTGAAGGTCTCAAACATGAT