Table 1.

Primers used for amplification and thermocycler conditions

DNA markers	Primers	Reference	Thermocycler conditions
trnL intron, trnL-F spacer	tab c (CGA AAT CGG TAG ACG CTA CG)	Taberlet et al. (1991)	Denaturation at 94 °C 2 min, 33 cycles with denaturation at 94 °C 1 min, annealing 54–62 °C 45 s, extension 72 °C 1 min 20 s and last extension 5 min
	tab f (ATT TGA ACT GGT GAC ACG AG)
atpB-rbcL spacer	atpB-1 (ACA TCK ART ACK GGA CCA ATA A)	Chiang et al. (1998)	Denaturation at 94 °C 2 min, 28 cycles with denaturation at 94 °C 1 min, annealing at 52 °C 1 min, extension at 72 °C 1 min and last extension 7 min
	rbcL-1 (AAC ACC AGC TTT RAA TCC AA)
trnH-psbA spacer	trnHGUG (CGC GCA TGG TGG ATT CAC AAT CC)	Shaw et al. (2005)	Denaturation at 80 °C, 33 cycles with denaturation at 94 °C 30 s, annealing at 50–56 °C 30 s, extension at 72 °C 1 min and last extension 10 min
	psbA (GTT ATG CAT GAA CGT AAT GCT C)
ITS rnDNA	26SE (ACG AAT TCA TGG TCC GGT GAA GTG TTC G)	Sun et al. (1994)	Denaturation at 94 °C 2 min, 28 cycles with denaturation at 94 °C 1 min, annealing at 52 °C 1 min, extension at 72 °C 1 min and last extension 7 min
	17SE (TAG AAT TCC CCG GTT CGC TCG CCG TTA C)
	(internal primers) ITS 2F (GCT GCG TTC TTC ATC GAT GC)	Kumar and Shukla (2005)
	ITS 3R (GCA TCG ATG AAG AAC GCA GC)