Table 2. Binding kinetics of anti-NS1 MAbs to dengue NS1 antigens determined by 1∶1 binding model.

Anti-NS1 mAb	NS1 antigen	ka [M−1s−1] ×104	kd [s−1] ×10−4	KD [nM]
Ab1	DENV1 NS1	N/A	N/A	N/A
	DENV2 NS1	2.1±0.04	2.4±0.06	11.5±0.5
	DENV3 NS1	1.6±0.20	14.8±4.9	96.5±4.3
	DENV4 NS1	2.1±0.02	1.1±0.05	51.0±2.1
Ab2	DENV1 NS1	4.8±0.05	2.59±0.04	5.4±0.1
	DENV2 NS1	2.8±0.06	3.54±0.03	12.3±0.2
	DENV3 NS1	N/A	N/A	N/A
	DENV4 NS1	N/A	N/A	N/A
Ab3	DENV1 NS1	1.4±0.03	1.6±0.03	11.9±0.5
	DENV2 NS1	1.9±0.01	1.3±0.04	6.9±0.2
	DENV3 NS1	1.1±0.02	4.7±0.02	41.6±1.1
	DENV4 NS1	1.2±0.00	3.7±0.07	31.2±0.6

K_D was calculated by k_d/k_a. Values are mean ± standard deviation from at least three experiments.