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. 2011 Jun 21;6(6):e20610. doi: 10.1371/journal.pone.0020610

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Al Saleh et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Panel A is a cartoon to illustrate agarose spot inside well with cells added some of which settle around the periphery. MCF7 (B) and pII (C, D) cells were plated into individual wells of a 12 well dish containing an agarose spot, and photographed after 24 h of incubation. MCF7 cannot penetrate even at high density whereas pII do so even at very low density (D). Arrows delineate the periphery of the agarose spot beneath. Scale bar represents 400 µm. This experiment was repeated at least ten times with identical outcome. The numbers of pII cells entering the agarose varied with density of cells settling at the periphery, and time.