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. 2011 Jun 21;6(6):e21221. doi: 10.1371/journal.pone.0021221

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Cervelló et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Results from PCR assay demonstrated the expression of undifferentiated genes in both cell lines (highlighted in figure by asterisk). In addition the absence of typical endoderm and ectoderm markers suggest their commitment to mesenchymal lineage throughout the presence of mesoderm genes. To assess the integrity of the samples analysed GAPDH gene expression was performed. ICEp: Epithelial somatic stem cell line; ICEs: Stromal somatic stem cell line; C+: hESC, human embryonic stem cell line (VAL-9) and C−: water. B. Normal karyotypes 46XX were obtained in both cases. C. Telomerase activity associated with length of telomeres was performed in order to known the undifferentiated status of the cell lines. Telomerase activity of positive control, VAL, showed a ladder of amplification products with six base increments starting at 50 nucleotides. An intermediate telomerase pattern was observed in both cases in comparison with hESC (VAL-9) and differentiated cell line (Fsk).