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. 2011 Jun 21;6(6):e21234. doi: 10.1371/journal.pone.0021234

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Zhang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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HEp-2 cells were incubated with (A) rHX2, (B) rHX1, and (C) PBS-BSA (negative control), and after washing, cells were incubated with rabbit antiserum to rHX2, antiserum to rHX1 and PBS-BSA, respectively. Then, HEp-2 cells were labeled with anti-rabbit IgG-FITC and examined by fluorescence microscopy.