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. 2011 Jun 21;6(6):e21370. doi: 10.1371/journal.pone.0021370

Figure 2. Cambogin triggers S phase arrest in Daoy cells via down regulation of cyclin A and E.

Figure 2

A. Cambogin induces S phase cell cycle arrest in Daoy cell line. Daoy cells were treated with cambogin for 48 h and followed by flow cytometry analysis. 1, control cells; 2-3, treatment with 2.5 and 5 µM cambogin, respectively. B. Cambogin inhibits DNA synthesis in Daoy cells using BrdU Incorporation Assay. 1, control Daoy cells; 2, Daoy cells treatment with cambogin at 5 µM for 24 h. C. Cambogin downregulates cyclin A and E using Western blotting. β-actin was used as internal loading control.