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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: J Immunol. 2011 May 23;187(1):350–360. doi: 10.4049/jimmunol.1004144

Figure 3.

Figure 3

Gp130 Y757F mice have lower levels of IFN-γ early after T. gondii infection. A) Kinetics of IFN-γ production in the serum of infected WT (n=10) or gp130 Y757F mice (n=11), as measured by ELISA. Data are pooled from 4 independent experiments, ***p<.001. B) At day 3 post-infection, whole splenocytes from WT and Y757F mice were stimulated with STAg for 48 hours and assayed for IFN-γ. Data are representative of 4 independent experiments. **p<.01. C) Real time PCR of total cellular RNA isolated from the livers of WT and Y757F mice at day 3 post-infection to detect IGTP, Lrg 47 and IGIP mRNA. Data are representative of two independent experiments. D) Flow cytometry of IL-17A vs. IFN-γ from splenocytes isolated from WT or gp130 Y757F mice at day 4 post-infection. Plots are gated on NK1.1+ CD3- cells; cells were incubated with PMA, Ionomycin and BFA for 4 hours before being stained. Total numbers of of IFN-γ+ (E) and IL-17+ (F) splenic NK cells from WT (n=5) and Y757F mice (n=7) at day 4 post-infection. Data are pooled from two independent experiments. *p<.05.