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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: J Immunol. 2011 May 23;187(1):350–360. doi: 10.4049/jimmunol.1004144

Figure 4.

Figure 4

Infection-induced T cell activation is normal in Y757F mice. A) Flow cytometry of splenocytes from WT or gp130 Y757F mice at day 8 post-infection showing expression of CD62L and CD44. Gated on CD3+ CD4+ cells. B) The number of CD44hi CD62Llow CD4+ T cells was enumerated in naïve WT and gp130 Y757F mice, and at day 4 and day 8 post-infection. n≥5 mice per time point. C) Flow cytometry of IFN-γ in splenocytes isolated from WT or gp130 Y757F mice at day 8 post-infection. Gated on CD3+ CD4+ events; cells were incubated with PMA, Ionomycin and BFA for 4 hours before being stained. D) Enumeration of IFN-γ+ CD4+ T cells in naïve or infected WT or gp130 Y757F mice. n≥5 mice per time point. E) ELISA of IFN-γ levels from cultures of splenocytes isolated at day 8 post-infection and incubated with STAg for 48 hours. Data are representative of 4 independent experiments.