Skip to main content
. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: J Immunol. 2011 Jun 1;187(1):47–54. doi: 10.4049/jimmunol.1002589

Fig. 2. Effect of CD14 gene silencing on hMPV-induced gene expression.

Fig. 2

MoDCs were transfected with 100 nM siRNA targeting CD14 (siCD14) or a scramble control (SiScr) for 48 h and infected with hMPV. Cells were harvested at 24 h p.i. to prepare total RNA for analysis of CD14, IL-8, RANTES and IFN-β gene expression by Q-RT-PCR (panel A) and to collect cell supernatants for measuring cytokines, chemokines, and IFN-β secretion by Bio-Plex or ELISA (panel B). Results are representative of two separate experiments. *, P < 0.05 relative to scramble control.