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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: J Immunol. 2011 May 27;187(1):482–489. doi: 10.4049/jimmunol.1003552

FIGURE 5. GX sPLA2 mediated hyperresponsiveness to LPS is reversed by plasma membrane cholesterol depletion and repletion.

FIGURE 5

(A) free cholesterol content of J774-C and J774-GX cells normalized to cell protein. Cells were incubated in the presence or absence of 10 mM MβCD at 37°C for 30 min to deplete cholesterol. Subsets of cholesterol-depleted cells were then incubated for 60 min with 1 mM MβCD -loaded cholesterol to replete cholesterol prior to LPS treatments. (B) TNFα in conditioned media from cells depicted in (A) after treatment with 100 ng/ml LPS for 8 h. (A, B) Data are means ± SEM (n > 3); **, p < 0.01; ***p < 0.001. The analysis of IL-6 secretion produced similar results.