FIGURE 6. Peripheral T cells in T cell-specific RASA1-deficient mice.

(A) Representative Western blot showing expression of RASA1 in CD4 and CD8 T cells isolated from spleens of littermate rasa1^fl/fl and rasa1^fl/fl plck-cre mice. (B) Representative flow cytometry plots of CD4 and CD8 expression (left) and mean number + 1 SEM of CD4 and CD8 T cells (right) in spleens of littermate rasa1^fl/fl and rasa1^fl/fl plck-cre mice (n=16 mice each genotype). (C) BM from rasa1^fl/fl plck-cre (CD45.2) and wild type C57BL/6 (+/+) (CD45.1/CD45.2) mice was adoptively transferred to lethally-irradiated wild type C57BL/6 (CD45.1) recipients. Flow cytometric plots show CD45, CD4 and CD8 expression in a single representative recipient spleen 6 wk after BM transfer. Numbers in CD45 plots indicate percentage of splenocytes of donor wild type or rasa1^fl/fl plck-cre origin among total splenocytes. Numbers in CD4/CD8 plots indicate percentage of cells in quadrants among splenocytes of the indicated genotype. Line graphs summarize results from 4 independent recipient mice. Percent CD4 and CD8 T cells among splenocytes of the indicated genotypes are shown. Alike symbols are used to represent data obtained from the same mouse within and between graphs. (D) At top are shown representative flow cytometry plots of CD44 and CD62L expression upon CD4 and CD8 T cells in spleens of littermate rasa1^fl/fl and rasa1^fl/fl plck-cre mice. Corresponding bar graphs below show mean cell number +1 SEM of the indicated splenic subpopulations (rasa1^fl/fl, n=6; rasa1^fl/fl plck-cre, n=7). Statistical significance was determined using unpaired (B and D) and paired (C) Students’ t-tests.