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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: J Immunol. 2011 May 25;187(1):538–552. doi: 10.4049/jimmunol.1100450

Figure 1. LDGs overexpress defensins and proinflammatory molecules.

Figure 1

A. Schematic representation of the sample group and gene list comparisons made using the gene microarray data (q-value <0.01, fold-change ≥1.5 and ≤ 0.7 for the up-regulated and down-regulated genes, respectively). Samples were matched-paired when comparing lupus neutrophils with autologous lupus LDGs (n=10 for each group). B. Lupus LDGs express elevated levels of azurophilic granule genes. Log base 2 mRNA mean expression values of five azurophil genes in control neutrophils (n=9), lupus neutrophils (n=10) and lupus LDGs (n=10): Myeloperoxidase (MPO), Elastase (ELANE), Defensin alpha 4 (DEFA4), Cathepsin G (CTSG) and azurocidin 1 (AZU1). Data are presented as mean ± SD. ***p<0.0001. C. Confirmation of enhanced mRNA expression by real-time PCR of various neutrophils genes in lupus LDGs when compared to autologous lupus neutrophils (n=7–12) and control neutrophils (n=7). Bar graph represents fold mRNA expression (mean ± SEM) after adjusting for housekeeping gene (GAPDH). *p<0.05 LDGs compared to control and/or autologous lupus neutrophils.