Figure 2.
Bacterial burden in (A) Peyer’s patches (PP), (B, C) spleen and (D, E) liver of BALB/c mice (n = 4–5 each group). Groups of mice were orally inoculated with χ3761(wild-type UK-1), χ9434(ΔpagP8), χ9732(ΔpagP81::Plpp lpxE), χ9485(ΔpagL7 ΔpagP8 ΔlpxR9) and χ9705(ΔpagL7 ΔpagP81::Plpp lpxE ΔlpxR9); 3–5 mice per group were euthanized, and spleen, liver and PP were collected, homogenized, and plated onto MacConkey and LB plates at 3 days and 6 days post inoculation. Salmonella colonies were counted and expressed as CFU per gram of PP, spleen, or liver. Significant differences in mean between groups were determined using one-way ANOVA analysis followed by Dunnett’s multiple comparison test. (* P < 0.05; ** P < 0.01).