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. Author manuscript; available in PMC: 2012 Jul 11.
Published in final edited form as: Brain Res. 2011 May 19;1400:31–41. doi: 10.1016/j.brainres.2011.05.028

Figure 5.

Figure 5

Effect of chronic food restriction on brain regional phosphorylation of AMPA receptor GluA1 subunit on Ser845 during a CPP conditioning session preceded by administration of cocaine or vehicle. Effects of chronic food restriction on GluA1 phosphorylation on Ser845 in dorsal hippocampus (top), nucleus accumbens (middle), and medial prefrontal cortex (bottom). Ad libitum fed (AL) and food-restricted (FR) rats were injected with cocaine (7.0 mg/kg, i. p.) or vehicle, immediately placed in one side compartment of a CPP apparatus and sacrificed following the 15-min conditioning session. Lysates were immunoblotted with anti-phospho-Ser845 GluA1 or anti-GluA1 antibodies. Following densitometry, intensities of bands corresponding to phospho-GluA1 for each subject were divided by the intensities of the corresponding total GluA1 bands to correct for small differences in protein loading. Results (mean ± S.E.M.) are expressed in comparison to the normalized control, which was defined as the AL group injected with vehicle. Representative blots (from top to bottom) correspond to phospho-Ser845 GluA1 and total GluA1. *FR greater than AL across drug treatments, p<.05. **FR treated with cocaine greater than all other groups, p at least <.05.