Table 1.
Proteins Identified in ESCC by MALDI-TOF MS
| spot no. | accession no. | identified proteins | score | no. of peptides | protein functions |
|---|---|---|---|---|---|
| 1 | gi|48735384 | Keratin 17 (KRT17) | 789 | 34 | Structural and binding protein |
| 2 | gi|32891807 | Biliverdin reductase B (BLVRB) | 236 | 9 | Converts biliverdin to bilirubin |
| 3 | gi|6470150 | Bip protein | 879 | 30 | Energy protein |
| 4 | gi|5453990 | Proteasome activator subunit 1 (PSME1) | 457 | 17 | Protein process |
| 5 | gi|4337097 | Chloride intracellular channel 1 (CLIC1) | 750 | 18 | Channel protein |
| 6 | gi|49456275 | Proteasome activator subunit 2 (PSME2) | 267 | 14 | Protein process |
| 7 | gi|21669669 | Immunoglobulin lambda light chain VLJ region | 471 | 8 | Antigen binding |
| 8 | gi|55925946 | Glutathione S-transferase omega 1 | 326 | 11 | Cell death/defense |
| 9 | gi|10835794 | Chain C, crystal structure of the Fab fragment of a human monoclonal IgM cold agglutinin | 264 | 10 | Antigen binding |
| 10 | gi|493940 | Carbonic anhydrase II complexed with the inhibitor Mts | 760 | 22 | Catalyzes reversible hydration of carbon dioxide |
| 11 | gi|15030212 | Glyoxalase 1 | 160 | 12 | Metabolism |
| 12 | gi|18204192 | Pituitary adenylate cyclase activating polypeptide (PACAP) | 516 | 10 | Stimulates adenylate cyclase and subsequently increases the cAMP level in target cells |
| 13 | gi|14249348 | Thioredoxin-like 5 | 276 | 6 | Activation of transcription factor |
| 14 | gi|7546412 | Manganese superoxide dismutase (Mn-SOD) | 594 | 12 | Detoxification |
| 15 | gi|55958715 | High mobility group box 1 (HMGB1) | 133 | 8 | Transcription/translation |
| 16 | gi|21669469 | Immunoglobulin kappa light chain VLJ region | 380 | 7 | Antigen binding |
| 17 | gi|15147369 | Cofilin 1 (nonmuscle) | 175 | 11 | Actin-depolymerizing factor |
| 18 | gi|2981750 | Chain F, secypa complexed with hagpia | 453 | 11 | Recognizing and binding site |
| 19 | gi|27923517 | Histone 3 | 106 | 6 | Involves with the structure of the nucleosomes of the `beads on a string' |
| 20 | gi|45767717 | Histone cluster 1, H2bm | 174 | 13 | DNA binding |
| 21 | gi|47077229 | Unknown protein | 147 | 13 | Unknown |
| 22 | gi|30584265 | Profilin 1 | 401 | 12 | Ubiquitous actin monomer-binding |
| 23 | gi|55662004 | FK506 binding protein 1A (FKBP1A) | 75 | 4 | Binding protein |
| 24 | gi|34616 | Beta-2 microglobulin | 96 | 5 | Major histocompatibility complex antigen |
| 25 | gi|1805696 | Polyubiquitin | 323 | 10 | Regulates many processes |
| 26 | gi|5902072 | Serine (or cysteine) proteinase inhibitor | 606 | 17 | Neutrophil elastase inhibitor |
| 27 | gi|109112231 | Heat shock protein 70 (HSP70) | 1110 | 32 | Protein folding |
| 28 | gi|62897681 | Calreticulin precursor variant | 563 | 16 | Protein folding |
| 29 | gi|6598323 | GDP dissociation inhibitor 2 | 566 | 23 | Regulate the GDP-GTP exchange reaction of members |
| 30 | gi|62898013 | F-actin capping protein alpha-1 subunit variant | 555 | 17 | Structural protein |
| 31 | gi|2780819 | Manganese-containing superoxide dismutase | 779 | 12 | Detoxification |
| 32 | gi|55959887 | Peroxiredoxin 1 (PRDX1) | 632 | 14 | Cell death/defense |
| 33 | gi|30377 | Keratin 13 (KRT13) | 96 | 6 | Intermediate filament |
| 34 | gi|55662177 | Translationally controlled tumor protein (TCTP) | 123 | 5 | A histamine-releasing factor, calcium binding protein |
| 35 | gi|1633065 | Carbonic anhydrase II mutant E117q, holo form | 178 | 9 | Reversible hydration of carbon dioxide |
| 36 | gi|179032 | Smooth muscle 22 (SM22) | 230 | 11 | A calponin-related protein |
| 37 | gi|7263012 | Cystatin B (CSTB) | 455 | 6 | Cysteine protease inhibitor |
| 38 | gi|19353393 | Tropomyosin 2 (TPM2) | 165 | 16 | Interact with Ras-related associated with diabetes, PDZ and LIM domain 7 and TPM1 |
| 39 | gi|55626262 | PREDICTED: similar to H2A histone family, member E | 204 | 6 | Involved with the structure of the nucleosomes of the `beads on a string' structure |
| 40 | gi|60835714 | Stratifin (SFN) | 191 | 8 | Stimulate the expression of matrix metallopeptidase 1 in fibroblasts |
| 41 | gi|442631 | Annexin 1 (ANX1) | 568 | 19 | Calcium ion binding |
| 42 | gi|20380766 | Carbonic anhydrase 1 (CA1) | 111 | 9 | Metalloenzyme |
| 43 | gi|13937950 | Triosephosphate isomerase 1 (TPI1) | 449 | 19 | Glycolytic enzyme |
| 44 | gi|1065006 | Unknown protein | 443 | 18 | Unknown |
| 45 | gi|62897565 | Transgelin variant (TAGLN) | 145 | 9 | Actin stress fibers |
| 46 | gi|2981750 | Chain F, secypa complexed with hagpia | 554 | 10 | Recognizing and binding site |
| 47 | gi|34783124 | Keratin 19 (KRT19) | 782 | 30 | Structural protein |