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. 2011 Jun 9;7(6):753–761. doi: 10.7150/ijbs.7.753

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Overexpression of LRRK2 variants has no direct effect on the transport activity of DAT heterogeneously expressed in dopaminergic cells. SH-SY5Y cells stably expressing GFP-DAT were seeded in 96-well plates and transiently transfected with indicated constructs. [³H]-DA uptake was determined 2 days after transfection. Data were analyzed according to the Michaelis-Menten kinetics using GraphPad Prism 4.0 software. The V_max and K_m values derived from the regression curves were not significantly different.