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Detecting PADI4 expression levels using real time PCR. Real time PCR measurement of PADI4 mRNA levels in ESCC (lane 1, n=5) and EAC (lane 2, n=5) samples. This expression was normalized to the expression of GAPDH. These results indicate that PADI4 transcription is significantly increased in ESCC and EAC tissues compared to that in the oesophageal paraneoplastic tissues. (p=0.0114 and 0.0133, respectively).
