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. Author manuscript; available in PMC: 2012 Jun 26.
Published in final edited form as: Neuron. 2011 Jun 23;70(6):1115–1127. doi: 10.1016/j.neuron.2011.04.028

Figure 2. Retinotopic map refinement, but not eye-specific segregation is rescued in the SC of β2(TG) mice.

Figure 2

(A, B) Focal DiI injections into dorsal retina result in a spot of label in the SC (whole mount, dorsal view). The target zone spot in β2(KO) mice and β2(TG) mice treated with doxycycline is much larger than in WT and β2(TG) mice. (C, D) Whole eye (vitreal) injections of Alexa-conjugated cholera toxin dye bulk label most RGC axon projections in the SC. Contralateral axons are green, ipsilateral red. Contralateral axons (green) project to the most superficial (SGS) layer of the SC (sagittal sections), ipsilateral eye axons (red) project to the SO layer just inferior to the contralateral axons. A large fraction of axons from the ipsilateral eye extend into the SGS layer in both β2(KO) and β2(TG) mice (D, top) and overlap with projections from the contralateral eye (D, bottom), indicating poor eye segregation. M, medial; C, caudal; R, rostral; SGS, stratum griseum superficial; SO, stratum opticum. Scale bars 500 μm for all figures. Biasterisk, P < 0.01 and triasterisk, P < 0.001, two-tailed Student’s t-test. Error bars are s.e.m.