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. Author manuscript; available in PMC: 2012 Jun 26.
Published in final edited form as: Neuron. 2011 Jun 23;70(6):1085–1099. doi: 10.1016/j.neuron.2011.04.025

Figure 4. PLC-γ and calcineurin mediate TrkA endocytosis.

Figure 4

(A) Calcineurin activity is required for NGF-dependent internalization of TrkA receptors. Cell surface biotinylation and TrkA immunoblotting shows that NGF-dependent TrkA endocytosis is reduced by calcineurin inhibiton. (B) Densitometric quantification of internalized TrkA, **p<0.01, n=6. (C) TrkA endocytosis is dependent on PLC-γ activity. PLC-γ inhibitor (U73122) decreases NGF-dependent TrkA endocytosis. (D) Densitometric quantification of internalized TrkA, *p<0.05 and **p<0.01, n=4. In (A) and (C), supernatants were probed for p85 for normalization.