Table 2.
Summary of the proteins identified by phosphoproteomics with aberrant phosphorylation in the AD versus control hippocampi. For each protein, the phosphorylation/protein expression values were averaged (n = 6) and expressed as fold phosphorylation compared to control. The p-value listed is the significance of altered phosphorylation levels relative to control samples with p< 0.05. Pb represents the probability of a false identity associated with each protein identification using the SEQUEST search algorithm. For the numbers of phosphorylated sites known we used Uniprot and Phosida databases, while for the number of phosphorylated sites predicted we used the NetphosK algorithm.
| PROTEIN | Swiss prot accession number |
Pb | # Of Peptides matched/s earched |
Fold AD/CTR |
P-Value | Number of phosphorylated sites: known/ (predicted) |
Biological process/molecular pathways |
|---|---|---|---|---|---|---|---|
| Alpha enolase | P06733 | 5e-010 | 4/4 | 2.50 | 0.033 | S 5/(8) T 2/(1) Y 3/(6) |
Glycolysis, energy metabolism |
| Gamma enolase | P09104 | 1e-014 | 6/6 | 29.6 | 0.038 | S 1/(6) T 0/(3) Y 2/(7) |
Glycolysis, Energy metabolism |
|
Glyceraldehyde-3- phosphate dehydrogenase |
P04406 | 3e-012 | 7/12 | 1.38 | 0.037 | S 6/(11) T 6/(3) Y 3/(4) |
Glycolysis, Energy metabolism, NO signals. |
| Creatine kinase B-type | P12277 | 4e-011 | 3/4 | 0.25 | 0.014 | S 3/(11) T 1/(6) Y 2/(4) |
Energy metabolism, creatine metabolic process |
|
NAD-dependent deacetylase sirtuin-2 |
Q8IXJ6 | 4e-012 | 6/9 | 0.63 | 0.049 | S 5/(14) T 0/(3) Y 0/(4) |
Cell cycle, Cell division, Mitosis |
|
Fructose-bisphosphate aldolase C |
P09972 | 1e-030 | 17/39 | 4.14 | 0.015 | S 1/(8) T 1/(4) Y 0/(2) |
Glycolysis, energy metabolism |
|
NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial |
O75489 | 2e-011 | 3/3 | 0.12 | 0.029 | S 0/(7) T 0/(5) Y 0/(2) |
Electron transport, Respiratory chain Transport |
|
6-phosphogluconate dehydrogenase, decarboxylating |
P52209 | 1e-030 | 9/11 | 10.1 | 0.001 | S 0/(13) T 0/(5) Y 0/(3) |
Cell anabolism. Pentose shunt, |
|
Glyoxylate reductase/hydroxypyruv ate reductase |
Q9UBQ7 | 1e-008 | 6/9 | 0.12 | 0.006 | S 0/(3) T 0/(6) Y 0/(1) |
Cell anabolism. glyoxylate metabolic process |
|
Dihydropteridine reductase |
P09417 | 4e-015 | 7/13 | 0.55 | 0.015 | S 0/(5) T 0/(4) Y 0/(0) |
Cell anabolism. Tetrahydrobiopterin biosynthesis, |
|
Glial fibrillary acidic protein |
P14136 | 1e-011 | 16/27 | 1.34 | 0.022 | S 0/(16) T 2/(5) Y 0/(3) |
Cell structure, astrocytic constituent of cytoskeleton |
|
Mitochondrial inner membrane protein |
Q16891 | 1e-011 | 10/14 | 0.20 | 0.035 | S 0/(32) T 0/(8) Y 0/(3) |
Cell structure, Mitochondrial structure |
|
Transitional endoplasmic reticulum ATPase |
P55072 | 2e-014 | 26/44 | 0.01 | 0.012 | S 14/(19) T 2/(9) Y 1/(3) |
Cellular trafficking, ER vesicles formation. |
|
Dihydropyrimidine related protein |
Q16555 | 8e-015 | 23/35 | 7.17 | 0.041 | S 5/(22) T 5/(7) Y 3/(7) |
Cell structure, Axonal growth, Differentiation, Neurogenesis |
| 1e-012 | 12/16 | 2.52 | 0.008 | ||||
| 1e-030 | 20/30 | 5.68 | 0.046 | ||||
|
Dual specificity mitogen activated protein kinase kinase 1 |
Q02750 | 5e-014 | 6/6 | 2.15 | 0.003 | S 4/(11) T 2/(4) Y 0/(1) |
Signaling. Activates ERK1 and ERK2 MAP kinases. |
|
Guanine nucleotide binding protein G(o) subunit alpha |
P09471 | 6e-015 | 11/22 | 16.4 | 0.050 | S 0/(10) T 0/(4) Y 0/(4) |
Signal Transducer |
|
Rab GDP dissociation inhibitor beta |
P50395 | 1e-030 | 22/34 | 0.61 | 0.024 | S 1/(9) T 0/(11) Y 1/(10) |
Signal transduction. Protein transport, regulation of GTPase activity, |