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Primers used for RT-PCR–HOB-related markers.
| gene | sequence (5′–3′) | melting temperature (°C) |
|---|---|---|
| GAPDH | F ACCCAGAAGACTGTGGATGG | 60 |
| R CAGTGAGCTTCCCGTTCAG | ||
| Runx-2 | F ATGCTTCATTCGCCTCAC | 60 |
| R ACTGCTTGCAGCCTTAAAT | ||
| OPN | F TTCCAAGTAAGTCCAACGAAAG | 60 |
| R GTGACCAGTTCATCAGATTCAT | ||
| collagen type I | F AGGGTCCCAACGAGATCGAGATCCG | 60 |
| R TACAGGAAGCAGACAGGGCCAACGTCG | ||
| BSP | F ATGGCCTGTGCTTTCTCAATG | 60 |
| R GGATAAAAGTAGGCATGCTTG |
