Figure 4.

Shift of the rprestin C_nonlin in response to mechanical stimulation. (A) C_nonlin measured in an excised outside-out patch before (gray trace) and during (black trace) application of 40 hPa to the back of the recording pipette. (B) V_1/2 values of C_nonlin determined from ramped phase-track recordings plotted as a function of time; application of 40 hPa is indicated by horizontal bars. Note the reversible shift in V_1/2 induced by the increase in membrane tension. (C) Pressure-induced shift in V_1/2 (ΔV_1/2) of rprestin C_nonlin is identical to the ΔV_1/2 obtained from OHCs. ΔV_1/2 for rprestin is given as mean ± SD from five experiments with 40-hPa pressure application to outside-out patches (■; given as mean pressure ± SD, see Materials and Methods); the ΔV_1/2 values obtained from outside-out patches of five OHCs at various pressure applications are given as individual data points (●). Line is fit of a regression line through the origin to the OHC data; slope of the line is 0.48 mV/hPa.