Fig. 4.

miRNA transcription provides information on the cellular state of CHO cell lines. (a) Cartoon depicting the biological relationship of sequenced CHO cell lines. (b) Unsupervised hierarchical clustering of CHO cell lines according to their miRNA transcription profiles identified 3 nodes, corresponding to serum-dependent K1 and DXB11 cell lines (1), serum-free adapted host and recombinant K1 cell lines (2), and serum-free host and recombinant DUXB11 cell lines (3). Principal component analysis of a miRNA expression matrix consisting of 6 samples (CHO cell lines) and 365 variables (conserved miRNAs) was centered and used for singular value decomposition using R. Principal components were retrieved, and biplot graphs were chosen for their illustration as PC1 versus PC2 (c) and PC2 versus PC3 (d).