Table 1.
Chinese hamster ovary cell lines and culture conditions.
| # | Library ID | Cell line ID | Description | Culture condition at total RNA harvest | Cell line collection | References |
|---|---|---|---|---|---|---|
| 1 | K1 fcs | CHO-K1 fcs | Host/5% serum/adherent | Exponential phase | ECACC CCL 61 | Tjio and Puck (1958) |
| 2 | K1 sf | CHO-K1 sf | Host/serum-free/suspension | Exponential phase | ECACC CCL 61 | Hernandez-Bort et al. (2010) |
| 3 | K1 rec | CHO-K1 (GS) | Recombinant/serum-free/suspension | Exponential phase | ECACC 85051005 | Jeffs et al. (2006) |
| 4 | DXB11 fcs | DUXB11 fcs | Host/5% serum/adherent | Exponential phase | ATCC CRL-9096 | Urlaub and Chasin (1980) |
| 5 | DXB11 sf | DUXB11 sf | Host/serum-free/suspension | Exponential phase | ATCC CRL-9096 | Lattenmayer et al. (2007) |
| 6 | DXB11 rec | EpoFc 14F2 | Recombinant/serum-free/suspension | Exponential phase | ATCC CRL-9096 | Lattenmayer et al. (2007) |
| 7.1 | Pool | CHO-K1 sf | Host/serum-free/suspension | heat shock (42 °C) | ECACC CCL 61 | See above |
| 7.2 | Pool | DUKXB11 fcs | Host/5% serum/adherent | Cold shock (33 °C) | ATCC CRL-9096 | See above |
| 7.3 | Pool | EpoFc 14F2 | Recombinant/serum-free/suspension | Heat shock (42 °C) | ATCC CRL-9096 | See above |
| 7.4 | Pool | CHO-K1 (GS) | Recombinant/serum-free/suspension | cold shock (33 °C) | ECACC 85051005 | See above |
| 7.5 | Pool | CHO-K1 fcs | Host/5% serum/adherent | Late stationary phase | ECACC CCL 61 | See above |
| 7.6 | Pool | DUKXB11 sf | Host/serum-free/suspension | late stationary phase | ATCC CRL-9096 | See above |
| 7.7 | Pool | EpoFc 14F2 | Recombinant/serum-free/suspension | Late stationary phase | ATCC CRL-9096 | See above |
| 7.8 | Pool | DUKXB11 sf | Host/serum-free/suspension | NaBu (2 mM 48 h) | ATCC CRL-9096 | See above |
| 7.9 | Pool | EpoFc 14F2 | Recombinant/serum-free/suspension | NaBu (2 mM 48 h) | ATCC CRL-9096 | See above |
sf, serum free; fcs, fetal calf serum; rec, recombinant; NaBu, sodium butyrate; GS, glutamine synthase selection system.