Figure 4.

A schematic representation of the targeted recombination procedure. In the first step, fMHV is created by homologous recombination of the felinized S gene (light gray) into the genomic RNA of the MHV‐A59 virus to generate a chimeric virus containing the felinized S gene, fMHV. In the second step, a mutant MHV‐A59 virus is formed by homologous recombination of the donor RNA containing additional mutations (denoted by the asterisk) in the original S gene with the genomic RNA of the felinized MHV. The dark gray region indicates the region of the genome into which a mutation may be inserted into MHV by this methodology.