Table 4.

Seven Plasmids for MHV‐A59‐1000, Their Backbones, Antibiotic Resistance, and Restriction Enzymes Used

Plasmid name	Vector backbone	Antibiotic resistance	Vector fragment (kpb)	Insert size (kpb)	RE to excise fragment
A	PCR‐XL‐TOPO (Invitrogen)	Kanamycin 30 µg/ml	3.5	4.9	MluI × BsmBI
B	pSMART‐LC (Lucigen)	Ampicillin 50 µg/ml	1.2 + 0.8	4.7	BglI × BsmBI
C	pSMART‐LC (Lucigen)	Ampicillin 50 µg/ml	1.2 + 1.5	2.0	BglI × BsmBI
D	pSMART‐LC (Lucigen)	Ampicillin 50 µg/ml	1.0 + 1.0	1.45	AhdI × BsmBI
E	pSMART‐LC (Lucigen)	Ampicillin 50 µg/ml	1.2 + 0.8	2.8	BglI × BsmBI
F	PCR‐XL‐TOPO (Invitrogen)	Kanamycin 30 µg/ml	3.5	7.0	BsmBI
G	pBR322	Ampicillin 50 µg/ml	2.2 + 2.1	8.7	SfiI × BsmBI

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