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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Neuropharmacology. 2011 Apr 16;61(3):524–543. doi: 10.1016/j.neuropharm.2011.04.008

Figure 3. Characteristics of the afterhyperpolarization of 5-HT and non-5-HT neurons across raphe subfield.

Figure 3

(A) Raw trace: Action potential trace with arrow indicating measurement points used to determine the properties of the AHP. (B) AHP Amplitude: 5-HT in the MR had the largest AHP amplitude (a vs. b), and dmDR 5-HT cells had the smallest (c vs. d) compared to all the other 5-HT neurons (a vs. b, c vs. d, different letters indicate significant differences between groups, p<0.05). AHP amplitude of non-5-HT neurons did not differ across subfields. AHP amplitude was generally greater in 5-HT cells than non-5-HT neurons (solid and dotted lines, p<0.01). Comparing cell types within individual subfields, 5-HT MR cells have a greater AHP amplitude than non-5-HT MR cells (ANOVA interaction, F(3,202)=4.45, p<0.001, Newman-Keuls posthoc, *p<0.05). (C) AHP t(1/2): 5-HT neurons did not differ across subfields. Non-5-HT neurons in the MR had a shorter AHP t(1/2) compared to lwDR and vmDR non-5-HT cells (a vs. b: different letter indicates statistical significance between groups, p<0.05). 5-HT cells have a longer AHP t(1/2) compared to non-5-HT neurons overall (solid and dotted lines, p<0.05). Comparing cell types within the individual subfields, dmDR and MR 5-HT neurons have a greater AHP t(1/2) than non-5-HT cells in the same subfield (*p<0.05). N=number of cells in each group.