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. 2010 Aug 30;16(12):3727–3736. doi: 10.1089/ten.tea.2010.0234

FIG. 2.

FIG. 2.

Comparison of BMP-2 expression, secretion, and activity after PEGDA encapsulation. Asterisks mark significant difference (p < 0.05) between AdBMP2-transduced cells and controls. (A) BMP-2 protein in culture supernatant taken from AdBMP2- or AdEmpty cassette-transduced cells (25000 VP/cell) (monolayer), or those encapsulated in PEGDA microspheres were quantified by sampling every other day for 9 days and evaluated using an ELISA. (B) AP activity in W20-17 cells after addition of conditioned media from AdBMP2- or AdEmpty-transduced cells (25000 VP/cell) (monolayer), or AdBMP2-transduced cells encapsulated in PEGDA microspheres. As a negative control, we also included culture supernatant from untransduced cells. AP activity is depicted as the average RLU, where n = 3. Error bars represent means ± SD for n = 3. A Student's t-test was applied to demonstrate significance. AdBMP2-transduced monolayer and microsphere groups had significantly greater AP activity and BMP-2 concentration levels (p < 0.05) when compared against other groups, but no differences when compared to each other. In other words, microencapsulation had no effect on BMP-2 release and function. AP, alkaline phosphatase; PEGDA, poly(ethylene glycol) diacrylate; RLU, relative chemiluminescence units.