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. 2001 Mar 20;98(7):4190–4195. doi: 10.1073/pnas.051634298

Figure 3.

Figure 3

Treatment with INF-γ plus TNF-α causes an increase in Cx43 immunoreactivity at contacts between rat microglia in culture and an increase abundance of Cx43. Cellular distribution of Cx43 was determined by immunofluorescence. (A) Distribution of Cx43 mmunofluorescence in a control culture at time 0. Most of the labeling was diffuse and localized to the perinuclear region. (B) In a culture treated with 1 ng/ml INF-γ plus 1 ng TNF-α for 9 h, there was marked labeling at intercellular appositions and reduced labeling in perinuclear regions. (Bar, 50 μm.) (C) The relative levels of Cx43 were determined by Western blot analysis of total homogenates of microglia (150 μg of protein per lane). Lane H is from a sample of rat heart to identify the mobility of different phosphorylation states of Cx43. NP is an unphosphorylated form, and P2 and P3 are two phosphorylated forms. The other lanes correspond to cultures of microglia treated with 1 ng/ml INF-γ plus 1 ng/ml TNF-α for 0, 2, 4, and 9 h.