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. Author manuscript; available in PMC: 2011 Jun 22.
Published in final edited form as: Cell Microbiol. 2009 Oct 27;12(3):343–361. doi: 10.1111/j.1462-5822.2009.01398.x

Figure 3.

Figure 3

Confocal microscopy of Legionella colonization and persistence in wild-type N2 nematodes. Wild-type worms were “pulsed” with mCherry-producing KB290 for three days and “chased” with GFP-producing KB130. Representative images after three days of exposure to KB130 are shown. (A) Bright-field image using DIC optics; (B) KB130 producing GPF in the intestinal and pharyngeal lumens; (C) KB290 producing mCherry in the intestinal lumen; and (D) merged images of A, B and C. Arrows demarcate the intestinal tract lumen; arrowhead indicates intact KB130 producing GFP in the pharyngeal lumen.