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. Author manuscript; available in PMC: 2011 Jun 22.
Published in final edited form as: Cell Microbiol. 2009 Oct 27;12(3):343–361. doi: 10.1111/j.1462-5822.2009.01398.x

Figure 9.

Figure 9

Elevated levels of germline apoptosis in C. elegans. (A) Germline apoptosis in C. elegans fed Legionella: (i) Number of corpse cells counted per gonad (n=10) plotted over time at 0, 12, 24, 36 hr in C. elegans N2 fed on E. coli OP50 (squares), L. pneumophila Philadelphia-1 type strain (triangles), L. longbeachae type strain (circles) and S. Typhimurium SL1344 type strain (diamonds); (ii) DIC image of corpse cells (indicated by arrows) in a gonad of a transgenic C. elegans WS2170 fed L. longbeachae for 36 hr with (iii) corresponding image of fluorescent actin filaments associated with corpse cells. Size marker is 10 μm. (B) Kaplan-Meier survival plot of C. elegans N2 fed L. pneumophila JR32 (squares, n=30) and the isogenic icmT mutant GS3011 (circles, n=44). P > 0.05 by pairwise comparison by the log-rank test. Data representative of three independent experiments. (C) Bar graph of mean corpse cell counts per gonad (n=20) in transgenic C. elegans WS2170 nematodes fed on the designated bacterial strain for 24 hr. P = 0.002 and P = 0.015 by pairwise comparison by the log-rank test of OP50 versus JR32 and Lp02, respectively.