Table 1.
Strains and plasmids used in this study.
| Strain or plasmid | Description (genotype and/or phenotype) | Reference or sourcea |
|---|---|---|
| Strains | ||
| Legionella pneumophila | ||
| Phildelphia-1 | Type strain (ATCC 33152) | CDC, Atlanta |
| SVir | Spontaneous L. pneumophila Philaldephia-1 Smr mutant | (Hoffman et al., 1989) |
| Lp02 | Philadelphia-1 derivative, rpsL hsdR thyA (Smr) mutant | (Berger et al., 1993) |
| KB130 | Lp02 (pKB127), Thy+ | (Morash et al., 2009) |
| KB290 | Lp02 (pKB288), Thy+ | This work |
| Lp03 | dotA03, thyA, hsdR, rpsL | (Berger et al., 1994) |
| Lp02(pKB9) | dotA complement | This study |
| JR32 | NaCl-sensitive isolate of L. pneumophila AM511 | (Sadosky et al., 1993) |
| GS3011 | JR32 icmT3011∷Km | (Segal et al., 1998) |
| Legionella longbeachae | ||
| ATCC 33462 | Type strain | Yousef Abu Kwaik |
| Escherichia coli | ||
| DH5α | F- Ф80dlacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 hsdR17 deoR thi-1 supE44 gyrA96 relA1 | Clonetech |
| OP50 | Uracil auxotrophy | CGC, (Brenner, 1974) |
| Salmonella enterica serovar Typhimurium | ||
| SL1344 | Type strain | Lab collection |
| SM022 | SL1344(pGP704:rpsM∷gfp) | Alejandro Aballay, (Vazquez-Torres et al., 1999) |
| Caenorhabditis briggsae | ||
| HK104 | Wild isolate from Okayama, Japan | CGC |
| Caenorhabditis elegans | ||
| Bristol N2 | Wild-type isolate from Bristol, England | CGC, (Brenner, 1974) |
| AB1 | Wild isolate from Adelaide, Australia | CGC |
| CB4555 | Wild isolate from Pasadena, California, USA | CGC |
| RC301 | Wild isolate from Freiburg, Germany | CGC |
| CB4856 | Wild isolate from Hawaii, USA | CGC |
| KU4 | sek-1(km4) | (Tanaka-Hino et al., 2002) |
| AU3 | nsy-1(ag3) | (Kim et al., 2002) |
| CB1370 | daf-2(e1370) | (Garsin et al., 2003) |
| daf-16(mgDf47) | (Garsin et al., 2003) | |
| GR1309 | daf-2(e1370); daf-16(mgDf47) | (Garsin et al., 2003) |
| tol-1(nr2033) | CGC | |
| PS3729 | unc-119(ed4); syIs78[ajm-1∷gfp + unc-119(+)] | CGC |
| WS1904 | unc-119(ed3); opEx813[Pced-10∷yfp∷actin + unc-119(+)] | Kinchen and Hengartner, unpublished |
| WS2170 | unc-119(ed3);opIs110[Pced-1∷YFP∷act-5;unc-119(+)] | (Kinchen et al., 2005) |
| Plasmid | ||
| pKB9 | dotA expression plasmid | (Roy et al., 1998) |
| pBH6119 | RSF1010 ori, promoterless, gfpmut3 tdΔi ; Thy+, Ampr | (Hammer et al., 1999) |
| pKB127 | pBH6119∷magA 245-bp promoter region cloned into BamHI and XbaI sites | (Morash et al., 2009) |
| pmCherry | pUC19∷mCherry, Ampr | Roger Y. Tsien, (Shaner et al., 2004) |
| pKB288 | PCR-amplified mCherry inserted into XbaI and SphI sites of pKB127 replacing gfpmut3 | This work |
a
ATCC, American Type Culture Collection; CDC, Centers for Disease Control and Prevention; CGC, Caenorhabditis Genetics Center.