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Overlay of 1H–15N HSQC spectra showing the trends of chemical shift changes upon PIP2-C4 titration. Only spectral regions around significantly perturbed residues, N44, E48, K49, D50, Q52, L104, and Y108, are enlarged. The black contours are peaks from the EIAV MA sample at a concentration of 195 μM. The red and blue contours represent the peaks from samples with protein:PIP2 molar ratios of 1:1 and 1:3, respectively.
