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Effects of microsecond to millisecond dynamics on R2 CPMG relaxation rates of EIAV MA in the presence of PIP2-C4. (A) Ratio of R2 measured at 0.5 ms (τcp) to R2 derived from the R1ρ data set collected at a spin lock strength of 2.5 kHz. (B) Ratio of R2 measured at 2 ms (τcp) to that at 0.5 ms (τcp). Residues with significant R2 enhancements are shown as empty circles. The protein concentration was 52.4 μM, and the PIP2-C4 concentration was 105 μM.
