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. Author manuscript; available in PMC: 2011 Jun 22.
Published in final edited form as: Neurobiol Dis. 2008 Jul 8;32(1):105–115. doi: 10.1016/j.nbd.2008.06.015

Figure 6. Induction of chaperones aids the processing and trafficking of PMP22.

Figure 6

(A) Sciatic nerve lysates (10μg/lane) from 6 month old Wt and C22 mouse (n=3) were treated with endo H (H) or PNGase F (N) and blotted with anti-PMP22 antibody. Total lysates (20μg/lane) from Wt (top) and neuropathic (bottom) DRG explants treated with DMSO or EC137 (50nM) were incubated with endo H (H) or PNGase F (N) and PMP22 was detected. R denotes endo H-resistant and S denotes endo H-sensitive fractions (%) of PMP22. (B) A representative blot for the levels of poly-ubiquitinated proteins in lysates (20μg/lane) of SCs from Wt and C22 mice treated with DMSO and EC137 (50 nM). (C) The levels of HSP70, HSP40, HSP27, αB-crystallin and HSP90 in SC lysates (20μg/lane) from Wt and C22 mice (n=3) treated with DMSO and EC137 (50nM) were assayed. (D) The levels of HSP70, HSP27 and αB-crystallin in DRG explants, and SC-depleted DRG neurons from Wt mice, after treatment with DMSO or EC137 (50nM, 48 h) are shown. GAPDH serves as a constitutive marker for the blots. Molecular mass in kDa.