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. Author manuscript; available in PMC: 2011 Jun 22.
Published in final edited form as: J Invest Dermatol. 2010 May 6;130(8):2017–2030. doi: 10.1038/jid.2010.108

Figure 6. PKCs α, ε, and η are not required for EGCG-dependent hINV promoter activity or morphology change.

Figure 6

(a) PKC α, ε, and η isoforms are not required for EGCG-stimulated hINV promoter activity. Keratinocytes were electroporated with 3 μg of control or PKC-δ-specific siRNA per 1 × 106 cells. At 48 hours after siRNA electroporation, cells were electroporated with 3 μg of endotoxin-free pINV-2473. After an additional 6 hours, the cells were treated with 40 μM EGCG and luciferase activity was assessed 18 hours later. The results are expressed as the mean±SD. This experiment is representative of four repeated experiments. (b) PKC α, ε, and η isoforms are not required for EGCG-dependent morphology change. Keratinocytes were electroporated with siRNA encoding the indicated PKC isoform and treated with EGCG for 48 hours as indicated above and morphology was assessed. The plots were generated by determining the percentage of cells containing large intracellular vacuoles or showing an elongated spindle-shaped morphology out of a minimum of 100 cells on each of three slides. The results are expressed as the mean±SD. This experiment is representative of four repeated experiments. EGCG, (-)-epigallocatechin-3-gallate; hINV, human involucrin; PKC, protein kinase C; siRNA, small interfering RNA; TPA, 12-O-tetradecanoylphorbol-13-acetate.