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. Author manuscript; available in PMC: 2011 Jun 22.
Published in final edited form as: J Invest Dermatol. 2008 Apr 3;128(9):2190–2197. doi: 10.1038/jid.2008.81

Figure 7. Increased phosphorylated PKCζ in membrane fractions of KCs stimulated with TNF.

Figure 7

(a) KCs were stimulated with 100 ng ml−1 of TNF-α for 0, 2, 4, 6, 8, and 10 minutes. While no significant change in the quantity of phospho-PKCζ was observed, pERK and pJNK were increased 6 minutes after TNF-α stimulation. (b) Increased phospho-PKCζ in the membrane and nuclear, but not in the cytosolic fractions, of primary KCs stimulated with TNF-α 100 ng ml−1 for 5 and 10 minutes (Student’s t-test). These blots are representations of three separate experiments. Values are mean ± SD, error bar = 1 SD.