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. 2001 Mar 20;98(7):4202–4208. doi: 10.1073/pnas.071054698

Figure 4.

Figure 4

Inhibition of purified ODC by SNAP, DEA-NO, NCX 4016 (NCX) and DFMO. DTT was separated from the enzyme by gel filtration through a Sephadex G-25 column. ODC was preincubated with the test agents, as indicated, at 37°C for 15 min. Enzyme mixture was then added to assay buffer containing 0.4 mM ornithine (labeled with 0.1 μCi l-[1-14C]ornithine; 55 Ci/mmol) and 40 μM pyridoxal 5′-phosphate. ODC activity was measured after 30-min incubation at 37°C as described (6). All concentrations of SNAP, DEA-NO, and DFMO tested inhibited ODC significantly (P < 0.05). NCX was not active (P > 0.05). CTL, control. Data represent means ± SE of duplicate determinations from four separate experiments.