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Effect of chronic morphine on the content of GRK2/3 protein in LMMP membranes (A) and Gβ immunoprecipitate (B). (C) The effect of chronic morphine on the content of Gβ in immunoprecipitate obtained using anti-Gβ antibody. Lanes 1 and 2 in all panels represent samples obtained from opioid naïve vs. chronic morphine-treated animals, respectively. Western analysis and immunoprecipitation were performed as described in Materials and Methods. Figure is representative of four experiments.
